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pDESTN-MTp-p53-Venus
(Plasmid #53736)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 53736 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pDEST R4-R3
  • Backbone manufacturer
    Gateway
  • Total vector size (bp) 6355
  • Modifications to backbone
    Neomycin resistance added for selection
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Top10
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    p53
  • Species
    H. sapiens (human)
  • Entrez Gene
    TP53 (a.k.a. BCC7, BMFS5, LFS1, P53, TRP53)
  • Promoter metallothionein promoter
  • Tag / Fusion Protein
    • Venus (C terminal on insert)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pDESTN-MTp-p53-Venus was a gift from Galit Lahav (Addgene plasmid # 53736 ; http://n2t.net/addgene:53736 ; RRID:Addgene_53736)

  • For your References section:

    Recurrent initiation: a mechanism for triggering p53 pulses in response to DNA damage. Batchelor E, Mock CS, Bhan I, Loewer A, Lahav G. Mol Cell. 2008 May 9;30(3):277-89. doi: 10.1016/j.molcel.2008.03.016. 10.1016/j.molcel.2008.03.016 PubMed 18471974
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