Skip to main content
Addgene

pMiR-HPV16-E7/E1-miR-375-m1-5
(Plasmid #53704)

Print

Full plasmid sequence is not available for this item.

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 53704 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pMiR-Target
  • Backbone manufacturer
    Origene
  • Backbone size w/o insert (bp) 7900
  • Total vector size (bp) 10152
  • Vector type
    Luciferase

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    HPV16-E7/E1
  • Species
    Human papillomavirus
  • Insert Size (bp)
    2252
  • Mutation
    Mutation in miR-375 binding sites m1-5 (refer to citation)
  • GenBank ID
    K02718

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoR1 (not destroyed)
  • 3′ cloning site Not1 (not destroyed)
  • 5′ sequencing primer AGAAGCTGCGCGGTGGTGTTGTG
  • 3′ sequencing primer CTGGAGGATCATCCAGCCGGCGT
    • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMiR-HPV16-E7/E1-miR-375-m1-5 was a gift from Edward Chan (Addgene plasmid # 53704 ; http://n2t.net/addgene:53704 ; RRID:Addgene_53704)

  • For your References section:

    miR-375 activates p21 and suppresses telomerase activity by coordinately regulating HPV E6/E7, E6AP, CIP2A, and 14-3-3zeta. Jung HM, Phillips BL, Chan EK. Mol Cancer. 2014 Apr 8;13(1):80. doi: 10.1186/1476-4598-13-80. 10.1186/1476-4598-13-80 PubMed 24708873
Related items: