CDKL5A-c014
(Plasmid
#53686)
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PurposeBaculovirus expression for structure determination; may not be full ORF
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 53686 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepFB-LIC-Bse
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Backbone manufacturerSGC
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Vector typeBaculovirus expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)Mach1
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Growth instructionsSf9 cell line is recommended for expression
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameCDKL5A
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Alt namegi|4507281
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MutationActivating mutations T169D / Y171E
- Promoter polyhedrin promoter
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Tag
/ Fusion Protein
- His6-TEV (N terminal on insert)
Cloning Information
- Cloning method Ligation Independent Cloning
- 5′ sequencing primer Polyhedrin forward (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Baculovirus transfer vector (Bac-to-bac), N-terminal His-tag, TEV http://www.thesgc.org/structures/4BGQ/
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
CDKL5A-c014 was a gift from Nicola Burgess-Brown (Addgene plasmid # 53686 ; http://n2t.net/addgene:53686 ; RRID:Addgene_53686)