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Addgene

INCENPA-c002
(Plasmid #53679)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 53679 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pGTvL1-SGC
  • Backbone manufacturer
    SGC
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Mach1
  • Growth instructions
    BL21(DE3)-R3-pRARE2 is recommended for expression
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    INCENPA
  • Mutation
    Bacterial expression for structure determination; may not be full ORF, see comments
  • Tag / Fusion Protein
    • GST-TEV (N terminal on insert)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

GST-TEV fusion vector, Tac promoter (IPTG inducible in any bacteria) http://www.thesgc.org/structures/4AF3/

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    INCENPA-c002 was a gift from Nicola Burgess-Brown (Addgene plasmid # 53679 ; http://n2t.net/addgene:53679 ; RRID:Addgene_53679)
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