CUL3A-c013
(Plasmid
#53672)
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Purposebacterial expression plasmid
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 53672 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepNIC-CTHF
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Backbone manufacturerSGC
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)Mach1
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Growth instructionsBL21(DE3)-R3-pRARE2 is recommended for expression
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameCUL3A
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Mutationsolubilizing mutations I342R & L346D
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Tag
/ Fusion Protein
- His6-TEV (C terminal on insert)
Cloning Information
- Cloning method Ligation Independent Cloning
- 5′ sequencing primer T7
- 3′ sequencing primer T7-term (Common Sequencing Primers)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
C-terminal His+flag, TEV-cleavable, for LIC cloning. EXP-06-AA9343 http://www.thesgc.org/structures/4APF/
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
CUL3A-c013 was a gift from Nicola Burgess-Brown (Addgene plasmid # 53672 ; http://n2t.net/addgene:53672 ; RRID:Addgene_53672)