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Addgene

ISPDA-c102
(Plasmid #53646)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 53646 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pFB-LIC-Bse
  • Backbone manufacturer
    SGC
  • Vector type
    Baculovirus expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Mach1 ; SF9
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    ISPDA
  • Alt name
    4CVH
  • Alt name
    gi|157412259
  • Insert Size (bp)
    1349
  • Promoter polyhedrin
  • Tag / Fusion Protein
    • His-6-TEV (N terminal on insert)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Baculovirus transfer vector (Bac-to-bac), N-terminal His-tag, TEV cleavable. http://www.thesgc.org/structures/4CVH/ Use BL21(DE3)-R3-pRARE2 strain for expression.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    ISPDA-c102 was a gift from Nicola Burgess-Brown (Addgene plasmid # 53646 ; http://n2t.net/addgene:53646 ; RRID:Addgene_53646)
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