DPYSL3A-c010
(Plasmid
#53641)
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PurposeBaculovirus expression for structure determination; may not be full ORF
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 53641 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepFB-6HZB
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Backbone manufacturerSGC
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Vector typeBaculovirus transfer vector
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)Mach1 ; SF9
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameDPYSL3A
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Alt name4BKN
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Insert Size (bp)1991
- Promoter polyhedrin promoter
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Tag
/ Fusion Protein
- His6-ZBasic-TEV (N terminal on insert)
Cloning Information
- Cloning method Ligation Independent Cloning
- 5′ sequencing primer Polyhedrin forward (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Baculovirus (Bac to bac). N-terminal His6-ZB-TEV tag. Clone using BseRI and standard Lic extensions http://www.thesgc.org/structures/4BKN/ Use BL21(DE3)-R3-pRARE2 strain for expression.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
DPYSL3A-c010 was a gift from Nicola Burgess-Brown (Addgene plasmid # 53641 ; http://n2t.net/addgene:53641 ; RRID:Addgene_53641)