SP100A-c082
(Plasmid
#53635)
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Purposebacterial expression plasmid
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 53635 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepSUMO-LIC
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Backbone manufacturerSGC
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)Mach1 ; BL21(DE3)-R3-pRARE2
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameSP100A
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Alt name4PTB
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Insert Size (bp)891
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MutationBacterial expression for structure determination; may not be full ORF, see comments
- Promoter T7
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Tag
/ Fusion Protein
- His-6-SUMO (N terminal on insert)
Cloning Information
- Cloning method Ligation Independent Cloning
- 5′ sequencing primer T7
- 3′ sequencing primer T7-term (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
T7/lac regulated, N-terminal His-SUMO tags, SUMO protease, LIC cloning using BsaI digest/T4 polymerase but non-standard LIC sites, SacB stuffer fragment. Optional non-cleavable biotinylation and His6 tags if reverse primer does not encode stop codon. NB. SUMO recognition sequence is a dummy entry. http://www.thesgc.org/structures/4PTB/ Use BL21(DE3)-R3-pRARE2 strain for expression.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
SP100A-c082 was a gift from Nicola Burgess-Brown (Addgene plasmid # 53635 ; http://n2t.net/addgene:53635 ; RRID:Addgene_53635)