CBSA-c032
(Plasmid
#53634)
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Purposebacterial expression plasmid
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 53634 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepNIC28-Bsa4
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Backbone manufacturerSGC
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)Mach1 ; BL21(DE3)-R3-pRARE2
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameCBSA
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Alt name4COO
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Alt namegi|4557415
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Insert Size (bp)1714
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MutationBacterial expression for structure determination; may not be full ORF, see comments
- Promoter T7
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Tag
/ Fusion Protein
- His-6-TEV (N terminal on insert)
Cloning Information
- Cloning method Ligation Independent Cloning
- 5′ sequencing primer T7
- 3′ sequencing primer T7-term (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
T7/lac regulated, N-terminal His-tag, TEV, LIC cloning using BsaI cleavage/T4 polymerase, SacB stuffer fragment, pET28 backbone. http://www.thesgc.org/structures/4COO/ Use BL21(DE3)-R3-pRARE2 strain for expression.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
CBSA-c032 was a gift from Nicola Burgess-Brown (Addgene plasmid # 53634 ; http://n2t.net/addgene:53634 ; RRID:Addgene_53634)