Skip to main content
Addgene

BLMA-c800
(Plasmid #53631)

Print

Full plasmid sequence is not available for this item.

Loading...

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 53631 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pNIC-NHT-CTB
  • Backbone manufacturer
    SGC
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Mach1 ; BL21(DE3)-R3-pRARE2
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    BLMA
  • Alt name
    4CGZ
  • Alt name
    gi|4557365
  • Insert Size (bp)
    2139
  • Mutation
    Bacterial expression for structure determination; may not be full ORF, see comments
  • Promoter T7
  • Tag / Fusion Protein
    • His-Tev (N terminal on insert)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

N-terminal His6, Tev, C-terminal TEV, Biotin; both cleaved by TEV http://www.thesgc.org/structures/4CGZ/ Use BL21(DE3)-R3-pRARE2 strain for expression.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    BLMA-c800 was a gift from Nicola Burgess-Brown (Addgene plasmid # 53631 ; http://n2t.net/addgene:53631 ; RRID:Addgene_53631)
Related items: