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Addgene

GLS2A-c007
(Plasmid #53621)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 53621 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pNIC28-Bsa4
  • Backbone manufacturer
    SGC
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Mach1 ; BL21(DE3)-R3-pRARE2
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    GLS2A
  • Alt name
    4BQM
  • Insert Size (bp)
    1075
  • Mutation
    Bacterial expression for structure determination; may not be full ORF, see comments
  • Promoter T7
  • Tag / Fusion Protein
    • His-6-TEV (N terminal on insert)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

T7/lac regulated, N-terminal His-tag, TEV, LIC cloning using BsaI cleavage/T4 polymerase, SacB stuffer fragment, pET28 backbone. http://www.thesgc.org/structures/4BQM/ Use BL21(DE3)-R3-pRARE2 strain for expression.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    GLS2A-c007 was a gift from Nicola Burgess-Brown (Addgene plasmid # 53621 ; http://n2t.net/addgene:53621 ; RRID:Addgene_53621)
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