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Addgene

Zebrafish ArcLight Q175
(Plasmid #53616)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 53616 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pCS2+
  • Backbone size w/o insert (bp) 5701
  • Total vector size (bp) 6955
  • Vector type
    Mammalian Expression
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Top10
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Zebrafish ArcLight-Q175
  • Alt name
    Zebrafish-Q175
  • Alt name
    Dr-VSD ArcLight Q175
  • Species
    D. rerio (zebrafish), Synthetic; Aequorea victoria
  • Insert Size (bp)
    1254
  • Mutation
    Dr-VSD contains R153Q mutation and an amino acid E (GAG) introduced immediately after starting M (ATG); Dr-VSP is truncated at Q176 (Q175 in the original Dr-VSP sequence) and super ecliptic pHluorin containing a A227D (FP numbering) mutation is fused to the C-terminal (after Q176).
  • GenBank ID
    NP_001020629.1 AY533296
  • Entrez Gene
    tpte (a.k.a. tpip, vsp, wu:fd20e11, wu:fi24b06)
  • Promoter CMV

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (not destroyed)
  • 3′ cloning site BgIII (destroyed during cloning)
  • 5′ sequencing primer AGAGGGTGAAGGTGATGCAACATAC
  • 3′ sequencing primer ACCTTCGGGCATGGCACTCTTG
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Zebrafish ArcLight Q175 was a gift from Vincent Pieribone (Addgene plasmid # 53616 ; http://n2t.net/addgene:53616 ; RRID:Addgene_53616)
  • For your References section:

    Fluorescent protein voltage probes derived from ArcLight that respond to membrane voltage changes with fast kinetics. Han Z, Jin L, Platisa J, Cohen LB, Baker BJ, Pieribone VA. PLoS One. 2013 Nov 27;8(11):e81295. doi: 10.1371/journal.pone.0081295. eCollection 2013. 10.1371/journal.pone.0081295 PubMed 24312287