pNatB (pACYCduet-naa20-naa25)
(Plasmid #53613)

• Purpose: Allows expression of the fission yeast NatB complex - chloramphenicol marker
• Depositing Lab: Dan Mulvihill
• Publication: Johnson et al PLoS One. 2010 Dec 23;5(12):e15801. doi: 10.1371/journal.pone.0015801.

Backbone

• Vector backbone: pACYCduet
• Backbone manufacturer: novagen
• Backbone size w/o insert (bp): 4008
• Total vector size (bp): 6699
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Chloramphenicol, 25 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert 1

• Gene/Insert name: naa20
• Species: S. pombe (fission yeast)
• Insert Size (bp): 543
• Entrez Gene: naa20 (a.k.a. SPCC16C4.12, nat3)
• Promoter: T7

Cloning Information for Gene/Insert 1

• Cloning method: Restriction Enzyme
• 5′ cloning site: Nde1 (not destroyed)
• 3′ cloning site: BamH1 (destroyed during cloning)
• 5′ sequencing primer: DuetUP2 Primer
• 3′ sequencing primer: T7 Terminator Primer

Gene/Insert 2

• Gene/Insert name: naa25
• Species: S. pombe (fission yeast)
• Insert Size (bp): 2436
• Entrez Gene: naa25 (a.k.a. SPBC1215.02c, arm1, mdm20)
• Promoter: T7

Cloning Information for Gene/Insert 2

• Cloning method: Restriction Enzyme
• 5′ cloning site: Sal1 (not destroyed)
• 3′ cloning site: Sal1 (not destroyed)
• 5′ sequencing primer: ACYCDuetUP1
• 3′ sequencing primer: DuetDOWN1 Primer

Resource Information

• Articles Citing this Plasmid:
  • 11 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

This plasmid contains an A2520G (Asp to Gly) mutation within naa25 insert. The depositor states that this mutation does not affect plasmid function.