psigma2-EGFP
(Plasmid #53610)

• Purpose: Expresses sigma2 of adaptor protein of AP2
• Depositing Lab: Tom Kirchhausen
• Publication: Kural et al Cell Rep. 2012 Nov 29;2(5):1111-9. doi: 10.1016/j.celrep.2012.09.025. Epub 2012 Oct 25.

Backbone

• Vector backbone: pEGFP-N1
• Backbone manufacturer: Clontech
• Backbone size w/o insert (bp): 4700
• Total vector size (bp): 5128
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: sigma2
• Alt name: adaptor-related protein complex 2, sigma 1 subunit
• Species: R. norvegicus (rat)
• Insert Size (bp): 426
• Entrez Gene: Ap2s1 (a.k.a. Ap17)
• Promoter: CMV
• Tag / Fusion Protein:
  • EGFP (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: HindIII (not destroyed)
• 3′ cloning site: BamHI (not destroyed)
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: EGFP-N

Resource Information

• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry