pMGIB-3xFLAG-reptin
(Plasmid
#53609)
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PurposeRetroviral expression of human reptin
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 53609 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepMGIB
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Backbone manufacturer-
- Backbone size w/o insert (bp) 6000
- Total vector size (bp) 7000
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Modifications to backboneRetroviral backbone. Insert followed by IRES-Blasticidin cassette
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Vector typeMammalian Expression, Retroviral
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Selectable markersBlasticidin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namereptin
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Alt nameRUVBL2
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Alt nameRuvB-like AAA ATPase 2
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SpeciesH. sapiens (human)
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MutationCDS mutated to confer resistance to shRNA. All mutations silent
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GenBank IDNM_006666.1; NP_006657.1
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Entrez GeneRUVBL2 (a.k.a. CGI-46, ECP-51, ECP51, INO80J, REPTIN, RVB2, TAP54-beta, TIH2, TIP48, TIP49B)
- Promoter LTR
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Tag
/ Fusion Protein
- 3xFLAG (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site AscI (not destroyed)
- 3′ cloning site PacI (not destroyed)
- 5′ sequencing primer T7 forward
- 3′ sequencing primer IRES-R (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pMGIB-3xFLAG-reptin was a gift from Steven Artandi (Addgene plasmid # 53609 ; http://n2t.net/addgene:53609 ; RRID:Addgene_53609) -
For your References section:
Identification of ATPases pontin and reptin as telomerase components essential for holoenzyme assembly. Venteicher AS, Meng Z, Mason PJ, Veenstra TD, Artandi SE. Cell. 2008 Mar 21;132(6):945-57. doi: 10.1016/j.cell.2008.01.019. 10.1016/j.cell.2008.01.019 PubMed 18358808
Map uploaded by the depositor.