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Addgene

pMGIB-3xFLAG-pontin
(Plasmid #53608)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 53608 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pMGIB
  • Backbone manufacturer
    -
  • Backbone size w/o insert (bp) 6000
  • Total vector size (bp) 7000
  • Modifications to backbone
    Retroviral backbone. Insert followed by IRES-Blasticidin cassette
  • Vector type
    Mammalian Expression, Retroviral
  • Selectable markers
    Blasticidin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    pontin
  • Alt name
    RUVBL1
  • Alt name
    RuvB-like AAA ATPase 1
  • Species
    H. sapiens (human)
  • Mutation
    pontin CDS mutated to confer resistance to shRNA. All mutations silent
  • GenBank ID
    NM_003707.2; NP_003698.1
  • Entrez Gene
    RUVBL1 (a.k.a. ECP-54, ECP54, INO80H, NMP 238, NMP238, PONTIN, Pontin52, RVB1, TIH1, TIP49, TIP49A)
  • Promoter LTR
  • Tag / Fusion Protein
    • 3xFLAG (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site AscI (not destroyed)
  • 3′ cloning site PacI (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer IRES-R
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMGIB-3xFLAG-pontin was a gift from Steven Artandi (Addgene plasmid # 53608 ; http://n2t.net/addgene:53608 ; RRID:Addgene_53608)

  • For your References section:

    Identification of ATPases pontin and reptin as telomerase components essential for holoenzyme assembly. Venteicher AS, Meng Z, Mason PJ, Veenstra TD, Artandi SE. Cell. 2008 Mar 21;132(6):945-57. doi: 10.1016/j.cell.2008.01.019. 10.1016/j.cell.2008.01.019 PubMed 18358808
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