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Addgene

pMGIB-3xFLAG-dyskerin
(Plasmid #53607)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 53607 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pMGIB
  • Backbone manufacturer
    -
  • Backbone size w/o insert (bp) 6000
  • Total vector size (bp) 7000
  • Modifications to backbone
    Retroviral backbone. Insert followed by IRES-Blasticidin cassette
  • Vector type
    Mammalian Expression, Retroviral
  • Selectable markers
    Blasticidin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    dyskerin
  • Alt name
    DKC1
  • Species
    H. sapiens (human)
  • GenBank ID
    isoform 1
  • Entrez Gene
    DKC1 (a.k.a. CBF5, CHINE1, DKC, DKCX, NAP57, NOLA4, XAP101)
  • Promoter LTR
  • Tag / Fusion Protein
    • 3xFLAG (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site AscI (not destroyed)
  • 3′ cloning site PacI (not destroyed)
  • 5′ sequencing primer T3
  • 3′ sequencing primer IRES-R
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMGIB-3xFLAG-dyskerin was a gift from Steven Artandi (Addgene plasmid # 53607 ; http://n2t.net/addgene:53607 ; RRID:Addgene_53607)

  • For your References section:

    A human telomerase holoenzyme protein required for Cajal body localization and telomere synthesis. Venteicher AS, Abreu EB, Meng Z, McCann KE, Terns RM, Veenstra TD, Terns MP, Artandi SE. Science. 2009 Jan 30;323(5914):644-8. doi: 10.1126/science.1165357. 10.1126/science.1165357 PubMed 19179534
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