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PurposeRetroviral expression of human dyskerin
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 53607 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepMGIB
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Backbone manufacturer-
- Backbone size w/o insert (bp) 6000
- Total vector size (bp) 7000
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Modifications to backboneRetroviral backbone. Insert followed by IRES-Blasticidin cassette
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Vector typeMammalian Expression, Retroviral
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Selectable markersBlasticidin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namedyskerin
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Alt nameDKC1
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SpeciesH. sapiens (human)
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GenBank IDisoform 1
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Entrez GeneDKC1 (a.k.a. CBF5, CHINE1, DKC, DKCX, NAP57, NOLA4, XAP101)
- Promoter LTR
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Tag
/ Fusion Protein
- 3xFLAG (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site AscI (not destroyed)
- 3′ cloning site PacI (not destroyed)
- 5′ sequencing primer T3
- 3′ sequencing primer IRES-R (Common Sequencing Primers)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pMGIB-3xFLAG-dyskerin was a gift from Steven Artandi (Addgene plasmid # 53607 ; http://n2t.net/addgene:53607 ; RRID:Addgene_53607) -
For your References section:
A human telomerase holoenzyme protein required for Cajal body localization and telomere synthesis. Venteicher AS, Abreu EB, Meng Z, McCann KE, Terns RM, Veenstra TD, Terns MP, Artandi SE. Science. 2009 Jan 30;323(5914):644-8. doi: 10.1126/science.1165357. 10.1126/science.1165357 PubMed 19179534
Map uploaded by the depositor.
![](https://media.addgene.org/data/easy-thumbnails/data/plasmids/53/53607/53607-map_mZTfBZa1x9lJ.pdf.940x940_q85_autocrop.png)