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Addgene

pIhh4.5k-Luc
(Plasmid #53603)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 53603 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    P4-Luc
  • Backbone manufacturer
    Generic
  • Backbone size w/o insert (bp) 6600
  • Total vector size (bp) 11100
  • Modifications to backbone
    None
  • Vector type
    Luciferase ; reporter

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Ihh promoter
  • Alt name
    Ihh4.5k
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    4500

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SmaI/EcoRI (destroyed during cloning)
  • 3′ cloning site NcoI (not destroyed)
  • 5′ sequencing primer Luc1
  • 3′ sequencing primer NA
    • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pIhh4.5k-Luc was a gift from Xiangli Yang (Addgene plasmid # 53603 ; http://n2t.net/addgene:53603 ; RRID:Addgene_53603)

  • For your References section:

    Atf4 regulates chondrocyte proliferation and differentiation during endochondral ossification by activating Ihh transcription. Wang W, Lian N, Li L, Moss HE, Wang W, Perrien DS, Elefteriou F, Yang X. Development. 2009 Dec;136(24):4143-53. doi: 10.1242/dev.043281. Epub 2009 Nov 11. 10.1242/dev.043281 PubMed 19906842
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