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Addgene

pHRIG-PRAS40
(Plasmid #53599)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 53599 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pHR-IG (pHR’tripCMV-IRES-eGFP)
  • Vector type
    Mammalian Expression, Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Stbl3
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    PRAS40
  • Alt name
    AKT1 substrate 1 (proline-rich)
  • Alt name
    AKT1S1
  • Species
    H. sapiens (human)
  • Mutation
    constitutively active (myristoylated version)
  • Entrez Gene
    AKT1S1 (a.k.a. Lobe, PRAS40)
  • Promoter CMV
  • Tags / Fusion Proteins
    • IRES-EGFP (C terminal on insert)
    • myc (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SacII (not destroyed)
  • 3′ cloning site PstI (not destroyed)
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer pCDH-rev
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

PRAS40 (pCMV5) was provided by Dr Richard Roth, Department of Chemical and Systems Biology, Stanford University.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pHRIG-PRAS40 was a gift from Heng Zhao (Addgene plasmid # 53599 ; http://n2t.net/addgene:53599 ; RRID:Addgene_53599)

  • For your References section:

    PRAS40 plays a pivotal role in protecting against stroke by linking the Akt and mTOR pathways. Xiong X, Xie R, Zhang H, Gu L, Xie W, Cheng M, Jian Z, Kovacina K, Zhao H. Neurobiol Dis. 2014 Jun;66:43-52. doi: 10.1016/j.nbd.2014.02.006. Epub 2014 Feb 27. 10.1016/j.nbd.2014.02.006 PubMed 24583056
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