pHRIG-Akt3
(Plasmid #53596)

• Purpose: lentiviral expression of constitutively active human Akt3 and EGFP
• Depositing Lab: Heng Zhao
• Publication: Xie et al J Cereb Blood Flow Metab. 2013 Dec;33(12):1875-85. doi: 10.1038/jcbfm.2013.132. Epub 2013 Aug 14.

Backbone

• Vector backbone: pHR-IG (pHR’tripCMV-IRES-eGFP)
• Vector type: Mammalian Expression, Lentiviral

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Stbl3
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: cAkt3
• Alt name: Akt3
• Alt name: v-akt murine thymoma viral oncogene homolog 3
• Species: H. sapiens (human)
• Mutation: constitutively active (myristoylated version)
• Entrez Gene: AKT3 (a.k.a. MPPH, MPPH2, PKB-GAMMA, PKBG, PRKBG, RAC-PK-gamma, RAC-gamma, STK-2)
• Promoter: CMV
• Tags / Fusion Proteins:
  • HA (C terminal on insert)
  • IRES-EGFP (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: SacII (not destroyed)
• 3′ cloning site: SacII (not destroyed)
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: pCDH-rev

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

cAkt3-HA (pcDNA3.1) provided by Dr Richard Roth, Department of Chemical and Systems Biology, Stanford University.

cAkt3 was fused with a Gag polypeptide and lacks the pleckstrin homology (PH) domain; the expression of a myristoylation signal enables Akt3 protein to attach to the cell membrane and become constitutively phosphorylated, thus activated (Aimee 1996).