pAtLCYbSK
(Plasmid #53588)

• Purpose: Produces an active Arabidopsis thaliana lycopene beta-cyclase enzyme in E. coli. Also known as pAt9SK.
• Depositing Lab: Francis X Cunningham Jr
• Publication: Cunningham FX et al Eukaryot Cell. 2007 Mar;6(3):533-45. Epub 2006 Nov 3.

Backbone

• Vector backbone: pBluescript SK-
• Backbone manufacturer: Stratagene
• Backbone size w/o insert (bp): 2958
• Total vector size (bp): 4748
• Modifications to backbone: The entire open reading frame of an Arabidopsis lcyB cDNA [GenBank U50739.1] in plasmid pZL1 (the original cDNA library plasmid) was excised as an EcoRI-HindIII fragment of ca. 1.8 kB and cloned in the same sites of pBluescript SK- so that the cDNA was fused, in frame, to the N terminal portion of the lacZ gene and under the control of the lac promoter.
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): XL1 Blue
• Growth instructions: Can be used with pAC-LYC or pAC-LYCipi to produce beta-carotene in E. coli strain TOP10. For this purpose, grow liquid cultures on a platform shaker at 28 degrees Celsius for 2-3 days in darkness, or grow on agar plates at room temperature for 3-7 days.
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: lcyB
• Alt name: lycopene beta-cyclase
• Species: A. thaliana (mustard weed)
• Insert Size (bp): 1946
• GenBank ID: U50739.1
• Entrez Gene: LYC (a.k.a. AT3G10230, LYCOPENE BETA-CYCLASE, lycopene cyclase)
• Promoter: lac

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: HindIII (not destroyed)
• 5′ sequencing primer: none
• 3′ sequencing primer: none

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry