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pCDNA-3xFLAG-NLS-TPP1
(Plasmid #53585)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 53585 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3.1
  • Backbone manufacturer
    Invitrogen/LIfe Technologies
  • Backbone size w/o insert (bp) 5500
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    TPP1
  • Alt name
    ACD
  • Alt name
    PTOP
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1632
  • Mutation
    Silent mutation to eliminate PacI site
  • GenBank ID
  • Entrez Gene
    ACD (a.k.a. PIP1, PTOP, TINT1, TPP1)
  • Promoter CMV
  • Tags / Fusion Proteins
    • 3xFLAG (N terminal on backbone)
    • NLS (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site AscI (not destroyed)
  • 3′ cloning site PacI (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer BGH reverse
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCDNA-3xFLAG-NLS-TPP1 was a gift from Steven Artandi (Addgene plasmid # 53585 ; http://n2t.net/addgene:53585 ; RRID:Addgene_53585)

  • For your References section:

    TPP1 OB-fold domain controls telomere maintenance by recruiting telomerase to chromosome ends. Zhong FL, Batista LF, Freund A, Pech MF, Venteicher AS, Artandi SE. Cell. 2012 Aug 3;150(3):481-94. doi: 10.1016/j.cell.2012.07.012. 10.1016/j.cell.2012.07.012 PubMed 22863003
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