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Purpose(Empty Backbone) To fuse your protein of interest to the N-terminus of BirA(R118G) and use in proximity-dependent biotin identification (BioID)
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Depositing Lab
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Publication
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 53581 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepcDNA3.1 MCS-BirA(R118G)-HA
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Backbone manufacturerKyle Roux, Addgene Plasmid 36047
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Modifications to backbonepcDNA3.1 MCS-BirA(R118G)-HA was cut with HpaI and ligated to a blunt ended selection cassette (Gateway conversion kit, cassette B) containing AttR sites flanking the ccdB gene and the chloramphenicol resistance gene.
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Vector typeMammalian Expression
- Promoter CMV
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Selectable markersNeomycin (select with G418)
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Tags
/ Fusion Proteins
- BirA(R118G) (C terminal on insert)
- HA tag (C terminal on insert)
Growth in Bacteria
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Bacterial Resistance(s)Chloramphenicol and Ampicillin, 25 & 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)ccdB Survival
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Copy numberHigh Copy
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer CMV-F
- 3′ sequencing primer BGH-rev (Common Sequencing Primers)
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
BirA(R118G)-HA destination vector was a gift from Karl Kramer (Addgene plasmid # 53581 ; http://n2t.net/addgene:53581 ; RRID:Addgene_53581)
