pCBFD2Bad
(Plasmid #53574)

• Purpose: Produces an active CBFD (carotenoid β-ring 4-dehydrogenase) enzyme in E. coli when expression is induced with arabinose
• Depositing Lab: Francis X Cunningham Jr
• Publication: Cunningham FX et al Plant Cell. 2011 Aug;23(8):3055-69. doi: 10.1105/tpc.111.086827. Epub 2011 Aug 23.

Backbone

• Vector backbone: pBad/His B
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 4092
• Total vector size (bp): 5111
• Modifications to backbone: The sequence ccATGgcagc was added to the Adonis aestivalis cbfd2 cDNA (AdKeto2) by PCR in order to provide first three codons that were missing from this cDNA, and also to introduce an NcoI site at the initiation codon. A 1.12 kb NcoI-XhoI fragment (with the XhoI site provided by the C terminal adaptor used in construction of the cDNA library) was then cloned in-frame in the NcoI and XhoI sites of pBAD/His B.
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): XL1 Blue
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: cbfd2
• Alt name: AdKeto2
• Alt name: carotenoid β-ring 4-dehydrogenase
• Species: Adonis aestivalis
• Insert Size (bp): 1122
• GenBank ID: AY644758.1
• Promoter: araBAD

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NcoI (not destroyed)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: none
• 3′ sequencing primer: none

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry