pCAndy-NBioTAP
(Plasmid
#53571)
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Purpose(Empty Backbone) Plasmid vector carrying N-Bio-1xproteinA tag
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 53571 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepCAndy
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Vector typeUnspecified
Growth in Bacteria
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Bacterial Resistance(s)Chloramphenicol, 25 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameNone
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site NotI (not destroyed)
- 3′ cloning site EcoRI (not destroyed)
- 5′ sequencing primer lac_promoter
- 3′ sequencing primer M13pUC-Fwd (Common Sequencing Primers)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCAndy-NBioTAP was a gift from Mitzi Kuroda (Addgene plasmid # 53571 ; http://n2t.net/addgene:53571 ; RRID:Addgene_53571) -
For your References section:
Reciprocal interactions of human C10orf12 and C17orf96 with PRC2 revealed by BioTAP-XL cross-linking and affinity purification. Alekseyenko AA, Gorchakov AA, Kharchenko PV, Kuroda MI. Proc Natl Acad Sci U S A. 2014 Feb 18;111(7):2488-93. doi: 10.1073/pnas.1400648111. Epub 2014 Feb 3. 10.1073/pnas.1400648111 PubMed 24550272