Chicken ArcLight S174
(Plasmid #53567)

• Purpose: Genetically encoded voltage sensor ArcLight with improved kinetics
• Depositing Lab: Vincent Pieribone
• Publication: Han et al PLoS One. 2013 Nov 27;8(11):e81295. doi: 10.1371/journal.pone.0081295. eCollection 2013.

Backbone

• Vector backbone: pCS2+
• Backbone size w/o insert (bp): 5701
• Total vector size (bp): 6955
• Vector type: Mammalian Expression
• Selectable markers: Hygromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Top10
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Chicken ArcLight-S174
• Alt name: Chicken-S174
• Alt name: Gg-VSD ArcLight S174
• Species: G. gallus (chicken), Synthetic; Aequorea victoria
• Insert Size (bp): 1251
• Mutation: Gg-VSP contains an R153Q mutation and an amino acid E (GAG) was introduced immediately after the start M (ATG); Gg-VSP is truncated at S175 (S174 in the native Gg-VSP sequence) and super ecliptic pHluorin containing a A227D (FP numbering) mutation is fused to the C-terminal (after S175).
• GenBank ID: XP_417079.2 AY533296
• Promoter: CMV

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: HindIII (not destroyed)
• 3′ cloning site: BamHI (not destroyed)
• 5′ sequencing primer: AGAGGGTGAAGGTGATGCAACATAC
• 3′ sequencing primer: ACCTTCGGGCATGGCACTCTTG

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The chicken voltage sensitive phosphatase (VSP) sequence used in this construct contains an E14K mutation, when compared to the reference sequence XP_417079.2 (the full plasmid sequence provided includes K14). This mutation could be polymorphism at the site or a PCR error. The depositing laboratory states that the plasmid functions as described in the associated publication.