pET28a(+) 6xHis-3xFlag-IFIT5
(Plasmid
#53561)
-
Purposebacterial expression of IFIT5
-
Depositing Lab
-
Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 53561 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
-
Vector backbonepET28a(+)
-
Backbone manufacturerNovagen
- Backbone size w/o insert (bp) 5369
-
Vector typeBacterial Expression
Growth in Bacteria
-
Bacterial Resistance(s)Kanamycin, 50 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert nameIFIT5
-
Alt nameinterferon-induced protein with tetratricopeptide repeats 5
-
SpeciesH. sapiens (human)
-
Entrez GeneIFIT5 (a.k.a. ISG58, P58, RI58)
- Promoter T7
-
Tags
/ Fusion Proteins
- 6xHIS (N terminal on backbone)
- 3xFLAG (N terminal on insert)
- TEV site (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site NheI (not destroyed)
- 3′ cloning site XhoI (not destroyed)
- 5′ sequencing primer T7
- 3′ sequencing primer T7 terminator (Common Sequencing Primers)
Resource Information
-
Article Citing this Plasmid
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
( Back to top)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pET28a(+) 6xHis-3xFlag-IFIT5 was a gift from Kathleen Collins (Addgene plasmid # 53561 ; http://n2t.net/addgene:53561 ; RRID:Addgene_53561) -
For your References section:
tRNA binding, structure, and localization of the human interferon-induced protein IFIT5. Katibah GE, Lee HJ, Huizar JP, Vogan JM, Alber T, Collins K. Mol Cell. 2013 Feb 21;49(4):743-50. doi: 10.1016/j.molcel.2012.12.015. Epub 2013 Jan 11. 10.1016/j.molcel.2012.12.015 PubMed 23317505