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pET28a(+) 6xHis-TEV-IFIT3
(Plasmid #53559)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 53559 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET28a(+)
  • Backbone manufacturer
    Novagen
  • Backbone size w/o insert (bp) 5369
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    IFIT3
  • Alt name
    interferon-induced protein with tetratricopeptide repeats 3
  • Species
    H. sapiens (human)
  • Entrez Gene
    IFIT3 (a.k.a. CIG-49, GARG-49, IFI60, IFIT4, IRG2, ISG60, P60, RIG-G, cig41)
  • Promoter T7
  • Tags / Fusion Proteins
    • 6xHIS (N terminal on backbone)
    • TEV (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NheI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer T7 terminal
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET28a(+) 6xHis-TEV-IFIT3 was a gift from Kathleen Collins (Addgene plasmid # 53559 ; http://n2t.net/addgene:53559 ; RRID:Addgene_53559)

  • For your References section:

    tRNA binding, structure, and localization of the human interferon-induced protein IFIT5. Katibah GE, Lee HJ, Huizar JP, Vogan JM, Alber T, Collins K. Mol Cell. 2013 Feb 21;49(4):743-50. doi: 10.1016/j.molcel.2012.12.015. Epub 2013 Jan 11. 10.1016/j.molcel.2012.12.015 PubMed 23317505
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