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Purposemammalian expression of IFIT3
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 53553 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepcDNA3.1
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Backbone manufacturerInvitrogen
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameIFIT3
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Alt nameinterferon-induced protein with tetratricopeptide repeats 3
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SpeciesH. sapiens (human)
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Entrez GeneIFIT3 (a.k.a. CIG-49, GARG-49, IFI60, IFIT4, IRG2, ISG60, P60, RIG-G, cig41)
- Promoter CMV
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Tag
/ Fusion Protein
- 3xFLAG (N terminal on insert)
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer CMV-F
- 3′ sequencing primer BGH Reverse (Common Sequencing Primers)
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pcDNA3.1 3xFlag IFIT3 was a gift from Kathleen Collins (Addgene plasmid # 53553 ; http://n2t.net/addgene:53553 ; RRID:Addgene_53553) -
For your References section:
tRNA binding, structure, and localization of the human interferon-induced protein IFIT5. Katibah GE, Lee HJ, Huizar JP, Vogan JM, Alber T, Collins K. Mol Cell. 2013 Feb 21;49(4):743-50. doi: 10.1016/j.molcel.2012.12.015. Epub 2013 Jan 11. 10.1016/j.molcel.2012.12.015 PubMed 23317505