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Addgene

pcDNA3.1 3xFLAG TPP1 88-249 delta166-172
(Plasmid #53550)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 53550 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3.1
  • Backbone manufacturer
    Invitrogen
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    TPP1
  • Alt name
    ACD
  • Alt name
    adrenocortical dysplasia homolog
  • Species
    H. sapiens (human)
  • Mutation
    contains aa 88-249, with aa 166-172 replaced with "GSSG"
  • GenBank ID
    NM_001082486.1
  • Entrez Gene
    ACD (a.k.a. PIP1, PTOP, TINT1, TPP1)
  • Promoter CMV
  • Tag / Fusion Protein
    • 3xFLAG (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NheI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer BGH Reverse
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3.1 3xFLAG TPP1 88-249 delta166-172 was a gift from Kathleen Collins (Addgene plasmid # 53550 ; http://n2t.net/addgene:53550 ; RRID:Addgene_53550)

  • For your References section:

    Specificity requirements for human telomere protein interaction with telomerase holoenzyme. Sexton AN, Youmans DT, Collins K. J Biol Chem. 2012 Oct 5;287(41):34455-64. doi: 10.1074/jbc.M112.394767. Epub 2012 Aug 14. 10.1074/jbc.M112.394767 PubMed 22893708
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