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Addgene

pBABE Hygro Flag hTERT
(Plasmid #53546)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 53546 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBABE hygro
  • Backbone manufacturer
    Bob Weinberg, Addgene plasmid #1765
  • Vector type
    Mammalian Expression, Retroviral
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    TERT
  • Alt name
    telomerase reverse transcriptase
  • Species
    H. sapiens (human)
  • Entrez Gene
    TERT (a.k.a. CMM9, DKCA2, DKCB4, EST2, PFBMFT1, TCS1, TP2, TRT, hEST2, hTRT)
  • Tag / Fusion Protein
    • FLAG (N terminal on insert)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBABE Hygro Flag hTERT was a gift from Kathleen Collins (Addgene plasmid # 53546 ; http://n2t.net/addgene:53546 ; RRID:Addgene_53546)

  • For your References section:

    The 5' guanosine tracts of human telomerase RNA are recognized by the G-quadruplex binding domain of the RNA helicase DHX36 and function to increase RNA accumulation. Sexton AN, Collins K. Mol Cell Biol. 2011 Feb;31(4):736-43. doi: 10.1128/MCB.01033-10. Epub 2010 Dec 13. 10.1128/MCB.01033-10 PubMed 21149580
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