pET15bLCFPT7
(Plasmid
#53545)
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PurposeActivation module. The plasmid consists of a mutant T7 RNA polymerase (T7 RNAP) activating its own expression via a T7 promoter carrying alac operator. Carbenicillin resistant
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 53545 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepET15b
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Backbone manufacturerNovagen
- Backbone size w/o insert (bp) 6000
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)Top10
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameT7 RNAP
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SpeciesT7 phage
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Insert Size (bp)2500
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pET15bLCFPT7 was a gift from Lingchong You (Addgene plasmid # 53545 ; http://n2t.net/addgene:53545 ; RRID:Addgene_53545) -
For your References section:
Temporal control of self-organized pattern formation without morphogen gradients in bacteria. Payne S, Li B, Cao Y, Schaeffer D, Ryser MD, You L. Mol Syst Biol. 2013 Oct 8;9:697. doi: 10.1038/msb.2013.55. 10.1038/msb.2013.55 PubMed 24104480
Map uploaded by the depositor.
