pOPINN-GFP
(Plasmid #53541)

• Purpose: pTirex based GFP fusion vector for expression in mammalian, E. coli and insect cells using the baculovirus system
• Depositing Lab: Ray Owens
• Publication: OPPF vectors (unpublished)

Backbone

• Vector backbone: pTriex-2
• Backbone manufacturer: EMDMillipore
• Backbone size w/o insert (bp): 6245
• Vector type: Mammalian Expression, Bacterial Expression, Insect Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Growth instructions: none
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: GFP
• Insert Size (bp): 710
• Tag / Fusion Protein:
  • N-His-GFP

Resource Information

• A portion of this plasmid was derived from a plasmid made by: EMDMillipore (formerly Merck)
• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please note: The pOPINN-GFP vector is not designed to express directly in insect cells i.e. by transfecting cells with the plasmid. However, the vector can be used as a transfer vector for the construction of baculoviruses which in turn can be used for expression insect cells. Please refer the primary publication for the pOPIN series of vectors for further information:

Berrow NS, Alderton D, Sainsbury S, Nettleship J, Assenberg R, Rahman N, Stuart DI, Owens RJ. A versatile ligation-independent cloning method suitable for high-throughput expression screening applications. Nucleic Acids Res. Feb 22; 2007;35(6):e45. Epub 2007.