pOPINEneo
(Plasmid
#53536)
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Purpose(Empty Backbone) pTriex-based expression vector which adds a C-his8 tag to the inserted gene
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 53536 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepTriex-2
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Backbone manufacturerEMDMillipore
- Backbone size (bp) 7049
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Vector typeMammalian Expression, Bacterial Expression, Insect Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Growth instructionsnone
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Copy numberHigh Copy
Cloning Information
- Cloning method Ligation Independent Cloning
Resource Information
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A portion of this plasmid was derived from a plasmid made byEMDMillipore (formerly Merck)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pOPINEneo was a gift from Ray Owens (Addgene plasmid # 53536 ; http://n2t.net/addgene:53536 ; RRID:Addgene_53536)