pOPINEneo-3C-GFP
(Plasmid #53534)

• Purpose: pTirex based GFP fusion vector for expression in mammalian, E. coli and insect cells using the baculovirus system
• Depositing Lab: Ray Owens
• Publication: OPPF vectors (unpublished)

Backbone

• Vector backbone: pTriex-2 neo
• Backbone manufacturer: EMDMillipore
• Backbone size w/o insert (bp): 6820
• Total vector size (bp): 7752
• Vector type: Mammalian Expression, Bacterial Expression, Insect Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Growth instructions: None
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: GFP
• Insert Size (bp): 710
• Tag / Fusion Protein:
  • GFP-His8 (C terminal on backbone)

Cloning Information

• Cloning method: Ligation Independent Cloning
• 5′ sequencing primer: TAA TAC GAC TCA CTA TAG GG

Resource Information

• A portion of this plasmid was derived from a plasmid made by: EMDMillipore (formerly Merck)
• Articles Citing this Plasmid:
  • 4 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry