pJL030
(Plasmid
#53533)
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Purposesupplies EtpC required for optimal EtpA expression on compatible plasmid
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 53533 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepACYC184
- Backbone size w/o insert (bp) 3719
- Total vector size (bp) 5567
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Vector typeBacterial Expression ; pACYC184
Growth in Bacteria
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Bacterial Resistance(s)Chloramphenicol, 25 μg/mL
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Growth Temperature37°C
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Growth Strain(s)Top10
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameetpC
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SpeciesE. coli
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Insert Size (bp)1900
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GenBank IDAY920525.2
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (not destroyed)
- 3′ cloning site NruI (not destroyed)
- 5′ sequencing primer none
- 3′ sequencing primer none (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please note that there is a L to M mutation in etpC. This mutation should not affect function.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pJL030 was a gift from James Fleckenstein (Addgene plasmid # 53533 ; http://n2t.net/addgene:53533 ; RRID:Addgene_53533) -
For your References section:
Purification of recombinant high molecular weight two-partner secretion proteins from Escherichia coli. Fleckenstein JM, Roy K. Nat Protoc. 2009;4(7):1083-92. doi: 10.1038/nprot.2009.87. Epub 2009 Jul 2. 10.1038/nprot.2009.87 PubMed 19707189