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pET-Gm1
(Plasmid #53528)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 53528 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET32a+
  • Backbone manufacturer
    Novagen
  • Backbone size w/o insert (bp) 5900
  • Total vector size (bp) 7696
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    For induction, grow at 23°C. Expression results largely in insoluble protein with a low yield of soluble, active enzyme
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    arginyl-tRNA synthetase
  • Alt name
    RARS
  • Species
    Glycin max (soybean)
  • Insert Size (bp)
    1794
  • GenBank ID
    FM209045
  • Promoter T7
  • Tags / Fusion Proteins
    • Thioredoxin (N terminal on backbone)
    • His Tag (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NcoI (not destroyed)
  • 3′ cloning site NcoI (not destroyed)
  • 5′ sequencing primer T7
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET-Gm1 was a gift from Gabor Igloi (Addgene plasmid # 53528 ; http://n2t.net/addgene:53528 ; RRID:Addgene_53528)

  • For your References section:

    Amino acid discrimination by arginyl-tRNA synthetases as revealed by an examination of natural specificity variants. Igloi GL, Schiefermayr E. FEBS J. 2009 Mar;276(5):1307-18. doi: 10.1111/j.1742-4658.2009.06866.x. 10.1111/j.1742-4658.2009.06866.x PubMed 19187230
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