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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 53522 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backboneN3
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameNPC1
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SpeciesM. musculus (mouse)
- Promoter CMV
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Tags
/ Fusion Proteins
- His6 (C terminal on backbone)
- ECFP (C terminal on backbone)
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer CMV-F
- 3′ sequencing primer EXFP-internal-R (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
NPC1 His6 ECFP was a gift from Matthew Scott (Addgene plasmid # 53522 ; http://n2t.net/addgene:53522 ; RRID:Addgene_53522) -
For your References section:
Anatomically defined neuron-based rescue of neurodegenerative Niemann-Pick type C disorder. Lopez ME, Klein AD, Dimbil UJ, Scott MP. J Neurosci. 2011 Mar 23;31(12):4367-78. doi: 10.1523/JNEUROSCI.5981-10.2011. 10.1523/JNEUROSCI.5981-10.2011 PubMed 21430138