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pUASp dsRed Rab5 CA
(Plasmid #53467)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 53467 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pUASp
  • Vector type
    Insect Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Rab05
  • Species
    D. melanogaster (fly)
  • Mutation
    Q88L
  • Entrez Gene
    Rab5 (a.k.a. Dmel_CG3664, AAF51265, BAA88244, CG3664, DRab5, Dm Rab5, DmRab5, Dmel\CG3664, Drab5, RAB5, RAB5a, Rab, Rab 5, Rab-5, dRab5, drab5, l(2)k08232, rab5)
  • Promoter GAL4
  • Tag / Fusion Protein
    • dsRed (N terminal on backbone)

Cloning Information

  • Cloning method Gateway Cloning
  • 5′ sequencing primer DsRed1-C
  • 3′ sequencing primer pUASp-3 (5'-AGGTTTAACCAGGGGATGCT-3')
    • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

This construct contains a SNP - G8C.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pUASp dsRed Rab5 CA was a gift from Matthew Scott (Addgene plasmid # 53467 ; http://n2t.net/addgene:53467 ; RRID:Addgene_53467)

  • For your References section:

    Thirty-one flavors of Drosophila rab proteins. Zhang J, Schulze KL, Hiesinger PR, Suyama K, Wang S, Fish M, Acar M, Hoskins RA, Bellen HJ, Scott MP. Genetics. 2007 Jun;176(2):1307-22. Epub 2007 Apr 3. 10.1534/genetics.106.066761 PubMed 17409086
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