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Purposeinteracts with MAPK signaling module
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 53458 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepcdna3
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Backbone manufacturerInvitrogen
- Backbone size w/o insert (bp) 5446
- Total vector size (bp) 10934
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)Top10
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namemitogen-activated protein kinase 8 interacting protein 3
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Alt nameJip3b
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Alt nameMapk8ip3
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SpeciesM. musculus (mouse)
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Insert Size (bp)5488
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GenBank IDAF178637.1
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Entrez GeneMapk8ip3 (a.k.a. D17Wsu15e, JIP-3, JSAP1, JSAP1a, JSAP1b, JSAP1c, JSAP1d, Jip3, Syd2, mKIAA1066)
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Tag
/ Fusion Protein
- Flag (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site Not1 (not destroyed)
- 5′ sequencing primer T7
- 3′ sequencing primer Sp6 (Common Sequencing Primers)
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pcdna3 Flag JIP3b was a gift from Roger Davis (Addgene plasmid # 53458 ; http://n2t.net/addgene:53458 ; RRID:Addgene_53458) -
For your References section:
Interaction of a mitogen-activated protein kinase signaling module with the neuronal protein JIP3. Kelkar N, Gupta S, Dickens M, Davis RJ. Mol Cell Biol. 2000 Feb;20(3):1030-43. PubMed 10629060