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pcdna3 T7 JIP3a
(Plasmid #53457)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 53457 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcdna3
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5446
  • Total vector size (bp) 10896
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Top10
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    mitogen-activated protein kinase 8 interacting protein 3
  • Alt name
    Jip3a
  • Alt name
    Mapk8ip3
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    5450
  • GenBank ID
    AF178636
  • Entrez Gene
    Mapk8ip3 (a.k.a. D17Wsu15e, JIP-3, JSAP1, JSAP1a, JSAP1b, JSAP1c, JSAP1d, Jip3, Syd2, mKIAA1066)
  • Tag / Fusion Protein
    • T7 (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoR1 (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer Sp6
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcdna3 T7 JIP3a was a gift from Roger Davis (Addgene plasmid # 53457 ; http://n2t.net/addgene:53457 ; RRID:Addgene_53457)

  • For your References section:

    Interaction of a mitogen-activated protein kinase signaling module with the neuronal protein JIP3. Kelkar N, Gupta S, Dickens M, Davis RJ. Mol Cell Biol. 2000 Feb;20(3):1030-43. PubMed 10629060
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