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Addgene

AtUBQ10+pLB12
(Plasmid #53449)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 53449 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pLB12
  • Backbone manufacturer
    Mark Curtis at ETH Zurich (Brand et al., Plant Physiology 2006)
  • Vector type
    plant expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Spectinomycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    UBQ10 promoter
  • Species
    A. thaliana (mustard weed); ecotype: Col
  • Insert Size (bp)
    1003
  • Entrez Gene
    UBQ10 (a.k.a. AT4G05320, C17L7.240, C17L7_240, UBI10, polyubiquitin 10, ubiquitin 10)
  • Promoter UBQ promoter
  • Tag / Fusion Protein
    • XVE (transcriptional activator) (C terminal on backbone)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The vector pLB12 contains both an activator unit and a b-glucuronidase (GUS) reporter within a responder unit.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    AtUBQ10+pLB12 was a gift from Anne Britt (Addgene plasmid # 53449)
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