Skip to main content
Addgene

pDONR-53BP1 1-1972 28A
(Plasmid #53447)

Print

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 53447 Standard format: Plasmid sent in bacteria as agar stab 1 $85 *
Add to Cart

* Log in to view industry pricing.

Backbone

  • Vector backbone
    Gateway pDONR 221
  • Backbone manufacturer
    Life Technologies
  • Backbone size w/o insert (bp) 4761
  • Total vector size (bp) 10677
  • Vector type
    Gateway

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    Stbl2
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    53BP1
  • Alt name
    siRNA resistant and ATM site-deficient construct of 53BP1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    5916
  • Mutation
    I914T mutation; All 28 SQ/TQ motifs in N-term of 53BP1 have been mutated to Ala; siRNA resistant mutation in 53BP1 is ACGCGCTGACGACCCGCTT
  • Entrez Gene
    TP53BP1 (a.k.a. 53BP1, TDRD30, p202, p53BP1)

Cloning Information

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pDONR-53BP1 1-1972 28A was a gift from Daniel Durocher (Addgene plasmid # 53447 ; http://n2t.net/addgene:53447 ; RRID:Addgene_53447)

  • For your References section:

    53BP1 is a reader of the DNA-damage-induced H2A Lys 15 ubiquitin mark. Fradet-Turcotte A, Canny MD, Escribano-Diaz C, Orthwein A, Leung CC, Huang H, Landry MC, Kitevski-LeBlanc J, Noordermeer SM, Sicheri F, Durocher D. Nature. 2013 Jul 4;499(7456):50-4. doi: 10.1038/nature12318. Epub 2013 Jun 12. 10.1038/nature12318 PubMed 23760478
Related items:
Commonly requested with: