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Addgene

pDONR-53BP1 1-1972 WT
(Plasmid #53446)

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Item Catalog # Description Quantity Price (USD)
Plasmid 53446 Standard format: Plasmid sent in bacteria as agar stab 1 $85 *
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Backbone

  • Vector backbone
    Gateway pDONR 221
  • Backbone manufacturer
    Life Technologies
  • Backbone size w/o insert (bp) 4761
  • Total vector size (bp) 10677
  • Vector type
    Gateway

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    Stbl2
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    53BP1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    5916
  • Mutation
    siRNA resistant and mutations A128V, T720A, A1347V, N1632S. Please see depositor's comments below.
  • Entrez Gene
    TP53BP1 (a.k.a. 53BP1, TDRD30, p202, p53BP1)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please note that mutations A128V, T720A, A1347V, N1632S in 53BP1 were found during Addgene's quality control. The depositor noted that these mutations do NOT affect plasmid function and 53BP1 functions as wild type.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pDONR-53BP1 1-1972 WT was a gift from Daniel Durocher (Addgene plasmid # 53446 ; http://n2t.net/addgene:53446 ; RRID:Addgene_53446)

  • For your References section:

    53BP1 is a reader of the DNA-damage-induced H2A Lys 15 ubiquitin mark. Fradet-Turcotte A, Canny MD, Escribano-Diaz C, Orthwein A, Leung CC, Huang H, Landry MC, Kitevski-LeBlanc J, Noordermeer SM, Sicheri F, Durocher D. Nature. 2013 Jul 4;499(7456):50-4. doi: 10.1038/nature12318. Epub 2013 Jun 12. 10.1038/nature12318 PubMed 23760478
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