pUAST Ag83b
(Plasmid
#53443)
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PurposeUAS-Anopheles gambiae Orco in pUAST Vector
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 53443 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepUAST
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Vector typeInsect Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert namepUAST Ag83b
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Alt nameOrco
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Alt nameOr83b
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Alt nameAgOr7
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SpeciesAnopheles gambiae
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Entrez GeneNEWENTRY
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site EcoRI (not destroyed)
- 5′ sequencing primer pCasper-hs (5'-GCAACTACTGAAATCTGCCAAG-3')
- 3′ sequencing primer SV40-splice-R2 (5'-CACAAACAATTAGAATCAGTAG-3') (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pUAST Ag83b was a gift from Leslie Vosshall (Addgene plasmid # 53443 ; http://n2t.net/addgene:53443 ; RRID:Addgene_53443)