RP125 pUAST EGFP:Orco
(Plasmid #53441)

• Purpose: N-terminal fusion of EGFP to Drosophila melanogaster Orco
• Depositing Lab: Leslie Vosshall
• Publication: Benton et al PLoS Biol. 2006 Feb;4(2):e20. Epub 2006 Jan 17.

Backbone

• Vector backbone: pUAST
• Vector type: Insect Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: EGFP:Orco
• Alt name: Or83b
• Alt name: Orco
• Alt name: Odorant receptor co-receptor
• Species: D. melanogaster (fly)
• Entrez Gene: Orco (a.k.a. Dmel_CG10609, 83A.2, 83b, A45, CG10609, DOR45, DOR83b, DmORCO, DmOrco, DmelOrco, Dmel\CG10609, OR49, OR83B, OR83b, ORCO, Or83b, OrCo, or83b, orco, vainsA)
• Tag / Fusion Protein:
  • EGFP (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (destroyed during cloning)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: EGFP-C (5'-CATGGTCCTGCTGGAGTTCGTG-3')
• 3′ sequencing primer: SV40-splice-R2 (5'-CACAAACAATTAGAATCAGTAG-3')

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry