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human CD40 promoter
(Plasmid #53433)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 53433 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pGL2 basic
  • Backbone manufacturer
    Promega
  • Backbone size w/o insert (bp) 5597
  • Total vector size (bp) 6300
  • Vector type
    Mammalian Expression, Luciferase

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    CD40 promoter
  • Alt name
    CD40 molecule, TNF receptor superfamily member 5
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    700
  • GenBank ID
    NM00125.4
  • Entrez Gene
    CD40 (a.k.a. Bp50, CDW40, TNFRSF5, p50)
  • Promoter CD40
  • Tag / Fusion Protein
    • luciferase (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site KpnI (not destroyed)
  • 3′ cloning site BglII (destroyed during cloning)
  • 5′ sequencing primer none
  • 3′ sequencing primer LucNRev
    • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    human CD40 promoter was a gift from Etty Benveniste (Addgene plasmid # 53433 ; http://n2t.net/addgene:53433 ; RRID:Addgene_53433)

  • For your References section:

    Critical role of tumor necrosis factor-alpha and NF-kappa B in interferon-gamma -induced CD40 expression in microglia/macrophages. Nguyen VT, Benveniste EN. J Biol Chem. 2002 Apr 19;277(16):13796-803. Epub 2002 Feb 5. 10.1074/jbc.M111906200 PubMed 11830590
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