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Addgene

RP78 pUAST Orco mRNA
(Plasmid #53431)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 53431 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pUAST
  • Vector type
    Insect Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Orco
  • Alt name
    Or83b
  • Alt name
    Odorant receptor co-receptor
  • Entrez Gene
    Orco (a.k.a. Dmel_CG10609, 83A.2, 83b, A45, CG10609, DOR45, DOR83b, DmORCO, DmOrco, DmelOrco, Dmel\CG10609, OR49, OR83B, OR83b, ORCO, Or83b, OrCo, or83b, orco, vainsA)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer pCasper-hs (5'-GCAACTACTGAAATCTGCCAAG-3')
  • 3′ sequencing primer SV40-splice-R2 (5'-CACAAACAATTAGAATCAGTAG-3')
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    RP78 pUAST Orco mRNA was a gift from Leslie Vosshall (Addgene plasmid # 53431 ; http://n2t.net/addgene:53431 ; RRID:Addgene_53431)

  • For your References section:

    Atypical membrane topology and heteromeric function of Drosophila odorant receptors in vivo. Benton R, Sachse S, Michnick SW, Vosshall LB. PLoS Biol. 2006 Feb;4(2):e20. Epub 2006 Jan 17. 10.1371/journal.pbio.0040020 PubMed 16402857
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