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Purposemammalian cell expression vector with HA-tag
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Depositing Lab
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Publication
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 53395 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepCG
- Backbone size w/o insert (bp) 5015
- Total vector size (bp) 5063
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameHA tag
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Insert Size (bp)48
- Promoter CMV
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site XbaI (destroyed during cloning)
- 3′ cloning site XbaI (not destroyed)
- 5′ sequencing primer CMV_for
- 3′ sequencing primer - (Common Sequencing Primers)
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCGN was a gift from Winship Herr (Addgene plasmid # 53395 ; http://n2t.net/addgene:53395 ; RRID:Addgene_53395) -
For your References section:
Differential transcriptional activation by Oct-1 and Oct-2: interdependent activation domains induce Oct-2 phosphorylation. Tanaka M, Herr W. Cell. 1990 Feb 9;60(3):375-86. 10.1016/0092-8674(90)90589-7 PubMed 2302733
Map uploaded by the depositor.
