pME-Gal4
(Plasmid #53366)

• Purpose: full-length native Gal4 with stop in Gateway middle entry clone
• Depositing Lab: Joshua Bonkowsky
• Publication: Fujimoto et al Dev Dyn. 2011 Oct;240(10):2324-34. doi: 10.1002/dvdy.22734. Epub 2011 Sep 8.

Backbone

• Vector backbone: pDONR221 (referred to as pME)
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 4762
• Total vector size (bp): 7408

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: native (full-length) Gal4
• Species: S. cerevisiae (budding yeast)
• Insert Size (bp): 2646
• Promoter: no promoter

Cloning Information

• Cloning method: Gateway Cloning
• 5′ sequencing primer: M13-F20
• 3′ sequencing primer: M13-rev

Resource Information

• A portion of this plasmid was derived from a plasmid made by: pME-Gal4 was cloned by PCR from pKG4021 (Guillemin et al., 2001; kind gift of M. Metzstein) using primers 5'-AGACCATGAAGCTACTGTCTTCTATCGA-3' and 5'-CTTACTCTTTTTTTGGGTTTGGTGG-3'.

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry